Analysis of RNA Metabolism in Fission Yeast

Jo Ann Wise; Olaf Nielsen

doi:10.1101/pdb.top079798

Analysis of RNA Metabolism in Fission Yeast

Cold Spring Harb Protoc. 2017 May 1;2017(5). doi: 10.1101/pdb.top079798.

Authors

Jo Ann Wise¹, Olaf Nielsen²

Affiliations

¹ Center for RNA Molecular Biology and Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106-4906 jaw17@case.edu.
² Department of Biology, Functional Genomics Division, University of Copenhagen, DK-2200 Copenhagen, Denmark.

PMID: 28461681
DOI: 10.1101/pdb.top079798

Abstract

Here we focus on the biogenesis and function of messenger RNA (mRNA) in fission yeast cells. Following a general introduction that also briefly touches on other classes of RNA, we provide an overview of methods used to analyze mRNAs throughout their life cycles.

MeSH terms

Protein Biosynthesis
RNA Splicing
RNA, Fungal / metabolism*
RNA, Messenger / metabolism
RNA, Ribosomal / metabolism
RNA, Small Interfering / metabolism
RNA, Small Nucleolar / metabolism
RNA, Transfer / metabolism
Schizosaccharomyces / genetics*

Substances

RNA, Fungal
RNA, Messenger
RNA, Ribosomal
RNA, Small Interfering
RNA, Small Nucleolar
RNA, Transfer