Assessment of NS1 gene-specific real time quantitative TaqMan PCR for the detection of Human Bocavirus in respiratory samples

Marco Ligozzi; Erica Diani; Federica Lissandrini; Rosanna Mainardi; Davide Gibellini

doi:10.1016/j.mcp.2017.04.005

Assessment of NS1 gene-specific real time quantitative TaqMan PCR for the detection of Human Bocavirus in respiratory samples

Mol Cell Probes. 2017 Aug:34:53-55. doi: 10.1016/j.mcp.2017.04.005. Epub 2017 Apr 27.

Authors

Marco Ligozzi¹, Erica Diani², Federica Lissandrini³, Rosanna Mainardi³, Davide Gibellini²

Affiliations

¹ Microbiology and Virology Unit, Department of Pathology and Diagnostic, University of Verona, Strada delle Grazie 8, 37134 Verona, Italy. Electronic address: marco.ligozzi@univr.it.
² Microbiology and Virology Unit, Department of Pathology and Diagnostic, University of Verona, Strada delle Grazie 8, 37134 Verona, Italy.
³ Microbiology and Virology Unit, AOUI "GB Rossi" Hospital Verona, Piazzale L.A. Scuro 10, 37134, Verona, Italy.

PMID: 28457983
DOI: 10.1016/j.mcp.2017.04.005

Abstract

Human Bocaviruses (HBoV) were associated with respiratory diseases. Here, we assessed a TaqMan^®-based PCR for the detection of all four HBoV subtype infections with a sensitivity up to 15 copies/reaction. To evaluate this assay on clinical samples, 178 nasopharyngeal aspirate specimens from pediatric cases were analyzed and HBoV genome was detected in 13 out of 178 patients with a viral load range between 1.6 × 10³ and 9.4 × 10⁷ copies/ml. These results indicated that this method could be used as an alternative technique for the diagnosis of HBoV infection.

Keywords: HBoV; Respiratory infection; qPCR.

MeSH terms

Child, Preschool
DNA, Viral / genetics*
Female
Human bocavirus / genetics*
Humans
Infant
Infant, Newborn
Male
Parvoviridae Infections / virology*
Real-Time Polymerase Chain Reaction / methods
Respiratory Tract Infections / virology*
Viral Load / methods

Substances

DNA, Viral