Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues

Mingguang Ren; Beibei Deng; Xiuqi Kong; Yonghe Tang; Weiying Lin

doi:10.1007/978-1-4939-6934-0_7

Preparation of a Two-Photon Fluorescent Probe for Imaging H₂O₂ in Lysosomes in Living Cells and Tissues

Methods Mol Biol. 2017:1594:129-139. doi: 10.1007/978-1-4939-6934-0_7.

Authors

Mingguang Ren¹, Beibei Deng¹, Xiuqi Kong¹, Yonghe Tang¹, Weiying Lin²

Affiliations

¹ Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, YiFu Building, No 336 West Road of Nan Xinzhuang, Jinan, Shandong, 250022, P. R. China.
² Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, YiFu Building, No 336 West Road of Nan Xinzhuang, Jinan, Shandong, 250022, P. R. China. weiyinglin2013@163.com.

PMID: 28456979
DOI: 10.1007/978-1-4939-6934-0_7

Abstract

Hydrogen peroxide (H₂O₂) plays important roles in many physiological and pathological processes. At the cellular organelle level, the abnormal concentrations of H₂O₂ in the lysosomes may cause redox imbalance and the loss of the critical functions of the lysosomes. Herein, we describe the preparation of a potent lysosome-targeted two-photon fluorescent probe (Lyso-HP) for the detection of H₂O₂ in the lysosomes in the living cells. This unique fluorescent probe can also be employed to effectively detect H₂O₂ in the living tissues using two-photon fluorescence microscopy.

Keywords: Fluorescence imaging; Fluorescent Probe; H2O2; Lysosome; Two photon.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Fluorescent Dyes
HeLa Cells
Humans
Hydrogen Peroxide / analysis*
Hydrogen Peroxide / metabolism
In Vitro Techniques
Lysosomes / metabolism*
Microscopy, Fluorescence / methods*

Substances

Fluorescent Dyes
Hydrogen Peroxide