Vitamin C and Vitamin E Protected B95-8 and Balb/c-3T3 Cells from Apoptosis Induced by Intermittent 50Hz ELF-EMF Radiation

Zhen Ding; Jintao Li; Fan Li; Mohammadreza Mohammadzad Mephryar; Shuicai Wu; Chen Zhang; Yi Zeng

Vitamin C and Vitamin E Protected B_95-8 and Balb/c-3T3 Cells from Apoptosis Induced by Intermittent 50Hz ELF-EMF Radiation

Iran J Public Health. 2017 Jan;46(1):23-34.

Authors

Zhen Ding¹, Jintao Li^{1

2}, Fan Li^{1

2}, Mohammadreza Mohammadzad Mephryar^{1

2}, Shuicai Wu¹, Chen Zhang³, Yi Zeng^{1

2}

Affiliations

¹ Beijing Key Laboratory of Environmental and Viral Oncology, School of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, 100124, China.
² State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China.
³ China Academy of Telecommunication Research of Ministry of Industry and Information Technology, Beijing, 100191, China.

PMID: 28451526
PMCID: PMC5401932

Abstract

Background: The extremely low-frequency electromagnetic field (ELF-EMF), mainly emitted by electric transmission lines and household electronic appliances, is becoming a worldwide health risk. It is imperative to investigate the biological impacts of ELF-EMF and to identify products that are resistant to the radiation from 50 Hz ELF-EMF. In this study, we investigated the biological impacts of apoptosis caused by 50 Hz Power line ELF-EMF and the protective effects of Vit C and Vit E.

Methods: We conducted this study in Beijing, China in 2013. B_95-8 and Balb/c-3T3 cells were divided into a sham group, an expo group and 3 expo groups in which the cells were preincubated with various concentrations of Vit C and Vit E. Then, all of the cells were exposed to 50 Hz Power line ELF-EMF and examined for apoptosis. The cells were collected for apoptosis detection after exposure.

Results: The percent of cells that undergoing apoptosis and preincubated with various concentrations of Vit C and Vit E were significantly lower than in the Expo group.

Conclusion: Vit C and Vit E exert significant protective effects from 50 Hz ELF-EMF radiation. The optimal protective concentrations of Vit C and Vit E are 10 μmol/L and 25 μmol/L, respectively. The protective effect of vitamins was more apparent for Balb/c-3T3 cells than B_95-8 cells.

Keywords: Apoptosis; Extremely low frequency; Vitamin C; Vitamin E.