[Expression of Death Associated Protein Kinase 1 in Chronic Lymphocytic Leukemia]

Guan-Ting Zhang; Xiao-Jing Liu; Lin Lu; Fei-Yue Zhao

doi:10.7534/j.issn.1009-2137.2017.02.003

[Expression of Death Associated Protein Kinase 1 in Chronic Lymphocytic Leukemia]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017 Apr;25(2):322-327. doi: 10.7534/j.issn.1009-2137.2017.02.003.

[Article in Chinese]

Authors

Guan-Ting Zhang¹, Xiao-Jing Liu², Lin Lu², Fei-Yue Zhao²

Affiliations

¹ Department of Clinical Laboratory Examination, The Affiliated Hospital of Henan Province Academy of Traditional Chinese Medicine, Zhengzhou 450004, Henan Province, China. E-mail:zhangguanting2008@163.com.
² Department of Clinical Laboratory Examination, The Affiliated Hospital of Henan Province Academy of Traditional Chinese Medicine, Zhengzhou 450004, Henan Province, China.

PMID: 28446269
DOI: 10.7534/j.issn.1009-2137.2017.02.003

Abstract

Objective: To explore the expression of death-associated protein kinase 1(DAPK1) in chronic lymphocytic leukemia(CLL).

Methods: The DAPK1 expression was studied by means of MEC1 cells and B lymphocytes from blood samples of the patients with CLL. The quantitative detection of mRNA and Western blot were used to detecte the expression of DAPK1 and autophagy-related genes at both mRNA and protein levels.

Results: mRNA quantitative detection and Western blot displayed that the DAPK1 expression in the patients with CLL was silenced. So, the expression of DAPK1 and autophagy related genes in MEC1 cells was not significantly different, no matter the cells were treated with or without INF-γ.

Conclusion: Scilencing of DAPK1 expression in CLL results in abnormality of autophagy behavior, thus leading to the occurence of disease.

MeSH terms

B-Lymphocytes
Death-Associated Protein Kinases / genetics*
Humans
Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
RNA, Messenger

Substances

RNA, Messenger
DAPK1 protein, human
Death-Associated Protein Kinases