Background: Genomic characterization of rotavirus (RoV) has not been adopted at large-scale due to the complexity of obtaining sequences for all 11 segments, particularly when feces are used as starting material.
Methods: To overcome these limitations, we developed a novel RoV capture and genome sequencing method combining commercial enzyme immunoassay plates and a set of routinely used reagents.
Results: Our approach had a 100% success rate, producing >90% genome coverage for diverse RoV present in fecal samples (Ct < 30).
Conclusions: This method provides a novel, reproducible and comparatively simple approach for genomic RoV characterization and could be scaled-up for use in global RoV surveillance systems.
Trial registration (prospectively registered): Current Controlled Trials ISRCTN88101063 . Date of registration: 14/06/2012.
Keywords: Antibody capture; Co-infection; Genome sequencing; Genomics; Phylogenetics; Reassortment; Rotavirus A.