Carbohydrate Analogue Microarrays for Identification of Lectin-Selective Ligands

Chembiochem. 2017 Jun 19;18(12):1077-1082. doi: 10.1002/cbic.201700091. Epub 2017 May 15.

Abstract

Fifty-five mono- and disaccharide analogues were prepared and used for the construction of microarrays to uncover lectin-selective ligands. The microarray study showed that two disaccharide analogues, 28' and 44', selectively bind to Solanum tuberosum lectin (STL) and wheat germ agglutinin (WGA), respectively. Cell studies indicated that 28' and 44' selectively block the binding of STL and WGA to mammalian cells, unlike the natural ligand LacNAc, which suppresses binding of both STL and WGA to cells.

Keywords: chemical biology; immobilization; lectins; microarrays; selective ligands.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbocyanines / chemistry
  • Carbohydrate Conformation
  • Disaccharides / chemistry
  • Disaccharides / pharmacology*
  • Dose-Response Relationship, Drug
  • Fluorescent Dyes / chemistry
  • HeLa Cells
  • Humans
  • Ligands
  • Microarray Analysis
  • Monosaccharides / chemistry
  • Monosaccharides / pharmacology*
  • Plant Lectins / antagonists & inhibitors
  • Plant Lectins / chemistry
  • Plant Lectins / isolation & purification
  • Plant Lectins / metabolism*
  • Protein Binding / drug effects
  • Solanum tuberosum / chemistry
  • Staining and Labeling
  • Triticum / chemistry
  • Wheat Germ Agglutinins / antagonists & inhibitors
  • Wheat Germ Agglutinins / chemistry
  • Wheat Germ Agglutinins / isolation & purification
  • Wheat Germ Agglutinins / metabolism*

Substances

  • Carbocyanines
  • Disaccharides
  • Fluorescent Dyes
  • Ligands
  • Monosaccharides
  • Plant Lectins
  • Wheat Germ Agglutinins
  • cyanine dye 3
  • potato lectin