Quantifying CBM Carbohydrate Interactions Using Microscale Thermophoresis

Haiyang Wu; Cédric Y Montanier; Claire Dumon

doi:10.1007/978-1-4939-6899-2_10

Quantifying CBM Carbohydrate Interactions Using Microscale Thermophoresis

Methods Mol Biol. 2017:1588:129-141. doi: 10.1007/978-1-4939-6899-2_10.

Authors

Haiyang Wu¹, Cédric Y Montanier¹, Claire Dumon²

Affiliations

¹ LISBP, Université de Toulouse, CNRS, INRA, INSA, 135 Avenue de, Rangueil, 31077, Toulouse Cedex 4, France.
² LISBP, Université de Toulouse, CNRS, INRA, INSA, 135 Avenue de, Rangueil, 31077, Toulouse Cedex 4, France. claire.dumon@insa-toulouse.fr.

PMID: 28417365
DOI: 10.1007/978-1-4939-6899-2_10

Abstract

MicroScale Thermophoresis (MST) is an emerging technology for studying a broad range of biomolecular interactions with high sensitivity. The affinity constant can be obtained for a wide range of molecules within minutes based on reactions in microliters. Here, we describe the application of MST in quantifying two CBM-carbohydrate interactions, a CBM3a toward cellulose nanocrystals and a CBM4 against xylohexaose.

Keywords: Binding studies; Fluorescence quenching; Fluorescent label; K d (dissociation constant); Microscale thermophoresis (MST).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Metabolism
Carbohydrates / chemistry*
Chemistry Techniques, Analytical / methods*
Clostridium / chemistry
Proteins / chemistry*
Proteins / metabolism
Temperature

Substances

Carbohydrates
Proteins