Laser-treated electrospun fibers loaded with nano-hydroxyapatite for bone tissue engineering

Javier Aragon; Nuria Navascues; Gracia Mendoza; Silvia Irusta

doi:10.1016/j.ijpharm.2017.04.022

Laser-treated electrospun fibers loaded with nano-hydroxyapatite for bone tissue engineering

Int J Pharm. 2017 Jun 15;525(1):112-122. doi: 10.1016/j.ijpharm.2017.04.022. Epub 2017 Apr 13.

Authors

Javier Aragon¹, Nuria Navascues¹, Gracia Mendoza², Silvia Irusta³

Affiliations

¹ Department of Chemical Engineering, Nanoscience Institute of Aragon (INA), University of Zaragoza, 50018, Zaragoza, Spain.
² Department of Chemical Engineering, Nanoscience Institute of Aragon (INA), University of Zaragoza, 50018, Zaragoza, Spain. Electronic address: gmmenc@unizar.es.
³ Department of Chemical Engineering, Nanoscience Institute of Aragon (INA), University of Zaragoza, 50018, Zaragoza, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN, 28029, Madrid, Spain. Electronic address: sirusta@unizar.es.

PMID: 28412451
DOI: 10.1016/j.ijpharm.2017.04.022

Abstract

Core-shell polycaprolactone/polycaprolactone (PCL/PCL) and polycaprolactone/polyvinyl acetate (PCL/PVAc) electrospun fibers loaded with synthesized nanohydroxyapatite (HAn) were lased treated to create microporosity. The prepared materials were characterized by XRD, FTIR, TEM and SEM. Uniform and randomly oriented beadless fibrous structures were obtained in all cases. Fibers diameters were in the 150-300nm range. Needle-like HAn nanoparticles with mean diameters of 20nm and length of approximately 150nm were mostly encase inside the fibers. Laser treated materials present micropores with diameters in the range 70-120μm for PCL-HAn/PCL fibers and in the 50-90μm range for PCL-HAn/PVAC material. Only samples containing HAn presented bioactivity after incubation during 30days in simulated body fluid. All scaffolds presented high viability, very low mortality, and human osteoblast proliferation. Biocompatibility was increased by laser treatment due to the surface and porosity modification.

Keywords: Bone regeneration; Electrospinning; Laser treatment; Nanohydroxyapatite; Scaffold.

MeSH terms

Biocompatible Materials / chemistry
Bone and Bones*
Cell Proliferation
Durapatite / chemistry*
Humans
Nanoparticles / chemistry*
Osteoblasts / cytology*
Polyesters
Polyvinyls
Tissue Engineering*
Tissue Scaffolds

Substances

Biocompatible Materials
Polyesters
Polyvinyls
polycaprolactone
polyvinyl acetate
Durapatite