Acetate-detoxification of wood hydrolysates with alkali tolerant Bacillus sp. as a strategy to enhance the lipid production from Rhodosporidium toruloides

Leonidas Matsakas; Katharina Novak; Josefine Enman; Paul Christakopoulos; Ulrika Rova

doi:10.1016/j.biortech.2017.04.002

Acetate-detoxification of wood hydrolysates with alkali tolerant Bacillus sp. as a strategy to enhance the lipid production from Rhodosporidium toruloides

Bioresour Technol. 2017 Oct:242:287-294. doi: 10.1016/j.biortech.2017.04.002. Epub 2017 Apr 4.

Authors

Leonidas Matsakas¹, Katharina Novak¹, Josefine Enman¹, Paul Christakopoulos¹, Ulrika Rova²

Affiliations

¹ Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, SE-971 87 Luleå, Sweden.
² Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, SE-971 87 Luleå, Sweden. Electronic address: ulrika.rova@ltu.se.

PMID: 28412146
DOI: 10.1016/j.biortech.2017.04.002

Abstract

The aim of the current work was to convert an acetate-rich hemicellulose liquid fraction (LF) from hot-water extraction of Betula pendula to oils for biodiesel, with Rhodosporidium toruloides. The toxicity of acetate was circumvented by biological detoxification with an isolated alkali-tolerant and acetate-resistant Bacillus sp. strain. Removal of other lignocellulose-derived inhibitors, such as furfural and phenols, was evaluated by two strategies; an activated carbon (AC) treatment of the undiluted LF, and dilution of the LF by 25% (0.75LF) and 50%. (0.50LF). The bacterium consumed most of the acetic acid in 6-8days in the treated or diluted media, which were subsequently used for cultivation of the yeast, for conversion of sugars to oils. The oil concentration reached 2.8 and 1.8g/L, in the AC LF and 0.75LF medium, respectively. In comparison, the oil accumulation in the same media without prior cultivation of Bacillus sp. was 0.86 and 0.03g/L, respectively.

Keywords: Acetate detoxification; Bacillus; Biodiesel; Birch; Rhodosporidium toruloides.

MeSH terms

Acetic Acid
Alkalies
Bacillus*
Basidiomycota*
Lipids*
Wood*

Substances

Alkalies
Lipids
Acetic Acid