Structural Characterization and Antifungal Studies of Zinc-Doped Hydroxyapatite Coatings

Molecules. 2017 Apr 9;22(4):604. doi: 10.3390/molecules22040604.

Abstract

The present study is focused on the synthesis, characterization and antifungal evaluation of zinc-doped hydroxyapatite (Zn:HAp) coatings. The Zn:HAp coatings were deposited on a pure Si (Zn:HAp_Si) and Ti (Zn:HAp_Ti) substrate by a sol-gel dip coating method using a zinc-doped hydroxyapatite nanogel. The nature of the crystal phase was determined by X-ray diffraction (XRD). The crystalline phase of the prepared Zn:HAp composite was assigned to hexagonal hydroxyapatite in the P63/m space group. The colloidal properties of the resulting Zn:HAp (xZn = 0.1) nanogel were analyzed by Dynamic Light Scattering (DLS) and zeta potential. Scanning Electron Microscopy (SEM) was used to investigate the morphology of the zinc-doped hydroxyapatite (Zn:HAp) nanogel composite and Zn:HAp coatings. The elements Ca, P, O and Zn were found in the Zn:HAp composite. According to the EDX results, the degree of Zn substitution in the structure of Zn:HAp composite was 1.67 wt%. Moreover, the antifungal activity of Zn:HAp_Si and Zn:HAp_Ti against Candida albicans (C. albicans) was evaluated. A decrease in the number of surviving cells was not observed under dark conditions, whereas under daylight and UV light illumination a major decrease in the number of surviving cells was observed.

Keywords: C. albicans; hydroxyapatite; thin layer; zinc.

MeSH terms

  • Antifungal Agents / chemistry*
  • Antifungal Agents / pharmacology
  • Candida albicans / drug effects
  • Coated Materials, Biocompatible / chemistry*
  • Coated Materials, Biocompatible / pharmacology
  • Durapatite / chemistry*
  • Durapatite / pharmacology
  • Microbial Sensitivity Tests
  • Particle Size
  • Structure-Activity Relationship
  • Surface Properties
  • X-Ray Diffraction
  • Zinc* / chemistry

Substances

  • Antifungal Agents
  • Coated Materials, Biocompatible
  • Durapatite
  • Zinc