[Generation of GABAergic interneuron-specific PGC-1α knockout mice]

Abstract

Objective: To generate mice which are specific for peroxisomproliferator-activated receptor-γ coactivator-1(PGC-1α) knockout in the GABAergic interneuron.

Methods: Conditional mice specific for PGC-1α^f/+ were introduced from the Jackson Laboratory, USA and initially inbred to obtain homozygote PGC-1α ^f/f mice. The PGC-1α^f/f conditional mice were further crossed with Dlx5/6-Cre-IRES-EGFP transgenic mice to achieve specific knockout of PGC-1α in the GABAergic interneuron.

Results: The offspring with specific knockout PGC-1α gene were successful for the generation of GABAergic interneuron, with the resulting genotype being PGC-1α ^{f/f;Dlx5/6-Cre-IRES-EGFP}.

Conclusion: The PGC-1α ^{f/f;Dlx5/6-Cre-IRES-EGFP} mice were obtained through a proper crossing strategy, which has provided a suitable platform for studying the function of PGC-1α in neuropsychiatric diseases.