Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real Samples

Mauro Tomassetti; Giovanni Merola; Elisabetta Martini; Luigi Campanella; Gabriella Sanzò; Gabriele Favero; Franco Mazzei

doi:10.3390/s17040819

Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real Samples

Sensors (Basel). 2017 Apr 10;17(4):819. doi: 10.3390/s17040819.

Authors

Mauro Tomassetti¹, Giovanni Merola², Elisabetta Martini³, Luigi Campanella⁴, Gabriella Sanzò⁵, Gabriele Favero⁶, Franco Mazzei⁷

Affiliations

¹ Department of Chemistry, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. mauro.tomassetti@uniroma1.it.
² Department of Chemistry, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. gancetto2@hotmail.it.
³ Department of Chemistry, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. elisabettamartini@libero.it.
⁴ Department of Chemistry, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. luigi.campanella@uniroma1.it.
⁵ Department of Chemistry and Pharmacy Technology, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. gabriella.sanzo@uniroma1.it.
⁶ Department of Chemistry and Pharmacy Technology, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. gabriele.favero@uniroma1.it.
⁷ Department of Chemistry and Pharmacy Technology, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. franco.mazzei@uniroma1.it.

Abstract

In this research, we developed a direct-flow surface plasmon resonance (SPR) immunosensor for ampicillin to perform direct, simple, and fast measurements of this important antibiotic. In order to better evaluate the performance, it was compared with a conventional amperometric immunosensor, working with a competitive format with the aim of finding out experimental real advantages and disadvantages of two respective methods. Results showed that certain analytical features of the new SPR immunodevice, such as the lower limit of detection (LOD) value and the width of the linear range, are poorer than those of a conventional amperometric immunosensor, which adversely affects the application to samples such as natural waters. On the other hand, the SPR immunosensor was more selective to ampicillin, and measurements were more easily and quickly attained compared to those performed with the conventional competitive immunosensor.

Keywords: ampicillin; analytical comparison; competitive amperometric immunosensor; flow surface plasmon resonance (SPR) immunosensor.

Publication types

Comparative Study

MeSH terms

Ampicillin
Anti-Bacterial Agents
Biosensing Techniques
Immunoassay
Surface Plasmon Resonance*

Substances

Anti-Bacterial Agents
Ampicillin