Stoichiometry and Turnover of the Stator and Rotor

Yusuke V Morimoto; Tohru Minamino

doi:10.1007/978-1-4939-6927-2_16

Stoichiometry and Turnover of the Stator and Rotor

Methods Mol Biol. 2017:1593:203-213. doi: 10.1007/978-1-4939-6927-2_16.

Authors

Yusuke V Morimoto¹, Tohru Minamino²

Affiliations

¹ Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita, Osaka, 565-0874, Japan. yvm010@riken.jp.
² Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan.

PMID: 28389956
DOI: 10.1007/978-1-4939-6927-2_16

Abstract

Fluorescence imaging techniques using green fluorescent protein (GFP) and related fluorescent proteins are utilized to monitor and analyze a wide range of biological processes in living cells. Stepwise photobleaching experiments can determine the stoichiometry of protein complexes. Fluorescence recovery after photobleaching (FRAP) experiments can reveal in vivo dynamics of biomolecules. In this chapter, we describe methods to detect the subcellular localization, stoichiometry, and turnovers of stator and rotor components of the Salmonella flagellar motor.

Keywords: FRAP; Flagellar motor; Fluorescence imaging; Fluorescent protein; Stoichiometry; Subcellular localization; Turnover.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / metabolism*
Flagella / metabolism*
Fluorescence Recovery After Photobleaching / methods
Green Fluorescent Proteins / metabolism
Molecular Motor Proteins / metabolism*
Photobleaching
Salmonella / metabolism

Substances

Bacterial Proteins
Molecular Motor Proteins
Green Fluorescent Proteins