[Preliminary study on the nucleic acid hybridization technic in detecting HSV-2 DNA corresponding sequence of cancer tissues of the uterine cervix]

H Y Cui; W Zhang; J Li; B Y Liu; J S Li; Y C Lin; W H Zhang; A R Wu; Z M Liu

[Preliminary study on the nucleic acid hybridization technic in detecting HSV-2 DNA corresponding sequence of cancer tissues of the uterine cervix]

Zhonghua Zhong Liu Za Zhi. 1987 Sep;9(5):342-4.

[Article in Chinese]

Authors

H Y Cui¹, W Zhang, J Li, B Y Liu, J S Li, Y C Lin, W H Zhang, A R Wu, Z M Liu

Affiliation

¹ Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Beijing.

PMID: 2838236

Abstract

In this paper, 34 samples, 25 from 18 cervical carcinoma patients and 9 from 8 non-cervical carcinoma patients, were assayed using (alpha-32P) dCTP HSV-2 DNA probe and nucleic acid hybridization technic. It was shown that the positive rates of HSV-2 DNA corresponding sequence were 50% (2/4), 36% (5/14) and 12% (1/8) in the in situ cancer, invasive cancer and non-cervical cancer tissues. No HSV was isolated from 7 cancer specimens which showed a positive reaction by nucleic acid hybridization.

Publication types

English Abstract

MeSH terms

Animals
Base Sequence*
Carcinoma in Situ / analysis
Carcinoma, Squamous Cell / analysis
DNA, Neoplasm / analysis*
DNA, Viral / analysis*
Female
Humans
Kidney / analysis
Male
Nucleic Acid Hybridization
Sequence Homology, Nucleic Acid*
Simplexvirus / genetics*
Uterine Cervical Neoplasms / analysis*

Substances

DNA, Neoplasm
DNA, Viral