Changes in blood anti-oxidation enzyme levels in MPTP-treated monkeys

Neurosci Lett. 2017 May 10:649:93-99. doi: 10.1016/j.neulet.2017.04.004. Epub 2017 Apr 2.

Abstract

Oxidative stress has been implicated in a range of neurodegenerative diseases, in particular, Parkinson's disease (PD). However, the pathogenic mechanism of its influence on PD progression remains unclear. In order to clarify this, PD models are generated by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intramuscular injection in eight rhesus monkeys. During the of progress PD pathogenesis, the activities of some major antioxidant enzymes, such as serum superoxide dismutase (SOD), glutathione peroxidase (GPX), and glutathione-s-transferase (GST), were measured, which show continuously decrease. While, the GSH and MDA content, as well as the expression of gamma-glutamylcysteine synthetase (GCS) and glutathione-s-transferase pi (GST-pi) exhibit robust increase. Taken together, this study shows the dynamic changes of antioxidant enzymes and lipid peroxidation in blood of PD monkeys during its progress, which will be important for the diagnosis and treatment of PD.

Keywords: Antioxidant; Behavior; MPTP; Monkey; Oxidative stress; Parkinson’s disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / administration & dosage
  • Animals
  • Glutathione Peroxidase / blood
  • Glutathione Transferase / blood
  • Injections, Intramuscular
  • Lipid Peroxidation
  • Macaca mulatta
  • Motor Activity / drug effects
  • Oxidative Stress*
  • Parkinsonian Disorders / blood*
  • Parkinsonian Disorders / enzymology*
  • Parkinsonian Disorders / pathology
  • Pars Compacta / drug effects
  • Pars Compacta / pathology
  • RNA, Messenger / blood
  • Superoxide Dismutase / blood

Substances

  • RNA, Messenger
  • 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Transferase