We have developed a chromium speciation and preconcentration method with the use of the graphite furnace atomic absorption spectrometry (GFAAS) technique. This method is based on single-drop microextraction (SDME) technique. Nowadays the microextractions have become popular, because low amount of organic solvent needs to be used for the separation. The sample was introduced into the extraction cell with a single chloroform droplet. For the separation and enrichment of chromium species, an ion-pair forming compound was used. After the extraction, the chromium content of the droplet was determined by GFAAS. The analytical sensitivity of the standard SDME technique was improved by increasing the volume of organic phase and by sample recirculation. Because of the increased contact area and the developed extraction device, the stability of droplet was markedly increased. As an application we have determined the r(VI) content of sea water by the GFAAS technique using these separation/enrichment methods. Under the optimized extraction conditions, the linear range, detection limit (S/N = 3) and precision (RSD, n = 3) for Cr(VI) were 0.14 - 5.00 μg/L, 0.042 μg/L, and ≤ 3.0%, respectively. The advantages of this method are the following: cost efficiency, the high enrichment of chromium species and easy usage with the GFAAS technique. Therefore the concentration of the chromium species can be determined at the ng/L level.