Telomere length profiles in primary human peritoneal mesothelial cells are consistent with senescence

Melisa Lopez-Anton; András Rudolf; Duncan M Baird; Laureline Roger; Rhiannon E Jones; Janusz Witowski; Donald J Fraser; Timothy Bowen

doi:10.1016/j.mad.2017.03.010

Telomere length profiles in primary human peritoneal mesothelial cells are consistent with senescence

Mech Ageing Dev. 2017 Jun:164:37-40. doi: 10.1016/j.mad.2017.03.010. Epub 2017 Apr 1.

Authors

Melisa Lopez-Anton¹, András Rudolf², Duncan M Baird³, Laureline Roger⁴, Rhiannon E Jones⁵, Janusz Witowski⁶, Donald J Fraser⁷, Timothy Bowen⁸

Affiliations

¹ Wales Kidney Research Unit, Division of Infection and Immunity, School of Medicine, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK. Electronic address: LopezAntonM@cf.ac.uk.
² Department of Pathophysiology, Poznan University of Medical Sciences, Poznan, Poland. Electronic address: arudolf@umed.poznan.pl.
³ Division of Cancer and Genetics, School of Medicine, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK. Electronic address: bairddm@cf.ac.uk.
⁴ Division of Cancer and Genetics, School of Medicine, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK. Electronic address: rogerl@cf.ac.uk.
⁵ Division of Cancer and Genetics, School of Medicine, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK. Electronic address: jonesr47@cf.ac.uk.
⁶ Department of Pathophysiology, Poznan University of Medical Sciences, Poznan, Poland. Electronic address: jwitow@ump.edu.pl.
⁷ Wales Kidney Research Unit, Division of Infection and Immunity, School of Medicine, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK; Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Museum Place, Cardiff CF10 3BG, UK. Electronic address: fraserdj@cf.ac.uk.
⁸ Wales Kidney Research Unit, Division of Infection and Immunity, School of Medicine, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK; Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Museum Place, Cardiff CF10 3BG, UK. Electronic address: bowent@cf.ac.uk.

PMID: 28373051
DOI: 10.1016/j.mad.2017.03.010

Abstract

Mesothelial cell (MC) senescence contributes to malignancy and tissue fibrosis. The role of telomere erosion in MC senescence remains controversial, with evidence for both telomere-dependent and telomere-independent mechanisms reported. Single telomere length analysis revealed considerable telomere length heterogeneity in freshly isolated human peritoneal MCs, reflecting a heterogeneous proliferative history and providing high-resolution evidence for telomere-dependent senescence. By contrast the attenuated replicative lifespan, lack of telomere erosion and induction of p16 expression in in vitro-aged cells was consistent with stress-induced senescence. Given the potential pathophysiological impact of senescence in mesothelial tissues, high-resolution MC telomere length analysis may provide clinically useful information.

Keywords: Mesothelium; Premature senescence; Single telomere length analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cellular Senescence / physiology*
Epithelial Cells / cytology
Epithelial Cells / metabolism*
Epithelium / metabolism
Humans
Peritoneum / cytology
Peritoneum / metabolism*
Telomere / metabolism*
Telomere Homeostasis / physiology*

Grants and funding

18246/CRUK_/Cancer Research UK/United Kingdom