The effective migration of Massilia sp. WF1 by Phanerochaete chrysosporium and its phenanthrene biodegradation in soil

Haiping Gu; Yuanzhi Chen; Xingmei Liu; Haizhen Wang; Jue Shen-Tu; Laosheng Wu; Lingzao Zeng; Jianming Xu

doi:10.1016/j.scitotenv.2017.03.205

The effective migration of Massilia sp. WF1 by Phanerochaete chrysosporium and its phenanthrene biodegradation in soil

Sci Total Environ. 2017 Sep 1:593-594:695-703. doi: 10.1016/j.scitotenv.2017.03.205. Epub 2017 Mar 28.

Authors

Haiping Gu¹, Yuanzhi Chen², Xingmei Liu², Haizhen Wang³, Jue Shen-Tu², Laosheng Wu⁴, Lingzao Zeng², Jianming Xu²

Affiliations

¹ Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou 310058, China; Department of Environmental Sciences, College of Forestry, Henan Agricultural University, Zhengzhou 450002, China.
² Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou 310058, China.
³ Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou 310058, China. Electronic address: wanghz@zju.edu.cn.
⁴ Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou 310058, China; Department of Environmental Sciences, University of California, Riverside, CA 92521, USA.

PMID: 28363181
DOI: 10.1016/j.scitotenv.2017.03.205

Abstract

Pollutant-degrading bacteria migrated by fungi may enhance the contacts between microorganisms and pollutants and improve the bioremediation efficiency of persistent organic pollutants in soil. Here, the migration of phenanthrene (PHE)-degrading bacteria Massilia sp. WF1 and Mycobacterium sp. WY10 by the hydrophobic fungi Phanerochaete chrysosporium (P. chrysosporium) and its effects on the PHE biodegradation in soil were investigated. Migration of the hydrophilic bacterium WF1 was better than that of the hydrophobic bacterium WY10 by P. chrysosporium mycelia since strain WF1 possesses flagellum and the type III secretion system. The interaction energy change of P. chrysosporium-WF1 was lower, but the interaction forces (van der Waals attractions, capillary forces, and cross-linking effects) were stronger than those of P. chrysosporium-WY10. Thus, the adhesive attraction between strain WF1 and P. chrysosporium was stronger, and consequently, strain WF1 was migrated by P. chrysosporium to a greater extent than WY10. The corresponding migration mechanism was inferred to be a bacterial 'passive' method: bacteria adhered to mycelia before they migrated with the growing mycelia. Moreover, migrated strain WF1 via P. chrysosporium showed effective PHE biodegradation in soil. Fungus-mediated migration of pollutant-degrading bacteria may play an important role in the bioremediation of pollutants in soil.

Keywords: Bioremediation; Fungus-bacterium interactions; Microbial migration; Phenanthrene.

MeSH terms

Bacterial Adhesion
Biodegradation, Environmental
Oxalobacteraceae / physiology*
Phanerochaete / metabolism*
Phenanthrenes / metabolism*
Soil
Soil Microbiology*
Soil Pollutants / metabolism*

Substances

Phenanthrenes
Soil
Soil Pollutants