Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection

Federica Bozzano; Francesco Marras; Andrea De Maria

doi:10.1007/978-1-4939-6872-5_14

Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection

Methods Mol Biol. 2017:1582:183-194. doi: 10.1007/978-1-4939-6872-5_14.

Authors

Federica Bozzano¹, Francesco Marras¹, Andrea De Maria²

Affiliations

¹ Molecular Immunology, University of Genova, Viale Benedetto XV 9, 16132, Genoa, Italy.
² Molecular Immunology, University of Genova, Viale Benedetto XV 9, 16132, Genoa, Italy. de-maria@unige.it.

PMID: 28357671
DOI: 10.1007/978-1-4939-6872-5_14

Abstract

Cytofluorimetric analysis is a typical method in immunology to evaluate phenotype and function of Natural Killer (NK) cells derived from HTLV-1/2 infected patients and healthy donors. Here, we described protocols to NK cells phenotypical and cytotoxicity assay, performed by flow cytometry on fresh and immune-magnetically or flow cytometry sorted NK cells. A new developed protocol able to evaluate IFNγ production has been included.

Keywords: HTLV-1; HTLV-2; Inhibitory receptors; NK; NK cell functional analysis.

MeSH terms

Female
Flow Cytometry / methods*
HTLV-I Infections / immunology*
HTLV-I Infections / pathology
HTLV-II Infections / immunology*
HTLV-II Infections / pathology
Human T-lymphotropic virus 1 / immunology*
Human T-lymphotropic virus 2 / immunology*
Humans
Immunity, Cellular*
Interferon-gamma / immunology
Killer Cells, Natural / immunology*
Killer Cells, Natural / pathology
Male

Substances

IFNG protein, human
Interferon-gamma