Spasmolytic and Antibacterial Activity of Two Citrus sinensis Osbeck Varieties Cultivated in Mexico

Amanda Sánchez-Recillas; Ana Ly Arroyo-Herrera; Jesús Alfredo Araujo-León; Emanuel Hernández Núñez; Rolffy Ortiz Andrade

doi:10.1155/2017/3960837

Spasmolytic and Antibacterial Activity of Two Citrus sinensis Osbeck Varieties Cultivated in Mexico

Evid Based Complement Alternat Med. 2017:2017:3960837. doi: 10.1155/2017/3960837. Epub 2017 Mar 5.

Authors

Amanda Sánchez-Recillas¹, Ana Ly Arroyo-Herrera¹, Jesús Alfredo Araujo-León², Emanuel Hernández Núñez³, Rolffy Ortiz Andrade¹

Affiliations

¹ Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán, Mérida, YUC, Mexico.
² Laboratorio de Cromatografía, Facultad de Química, Universidad Autónoma de Yucatán, Mérida, YUC, Mexico.
³ CONACYT, Departamento de Recursos del Mar, CINVESTAV-Unidad Mérida, Mérida, YUC, Mexico.

Abstract

Antibacterial activity on ATCC strains of Escherichia coli, Salmonella enterica, Salmonella enteritidis, and Salmonella choleraesuis and spasmolytic effect on contraction on rat ileum trips were determinate. Eight organic extracts (hexanic and methanolic) of albedo (mesocarp) and flavedo (pericarp) of two varieties (Valencian and National) of Citrus sinensis (L.) Osbeck of Yucatán, México, were studied. Additionally, chromatographic fingerprints were obtained and correlated with their pharmacological effects. MAN, MAV, and HFN extract caused inhibition against S. choleraesuis (MIC: 1000 µg/mL) and S. enteritidis (MIC: 1000 µg/mL). Regarding the spasmolytic effect, the Valencian extracts variety was more efficient on spontaneous contraction, HAV (E_max = 51.98 ± 1.98%), MAV (E_max = 35.98 ± 1.42%), HFV (E_max = 68.91 ± 4.14%), and MFV (E_max = 51.28 ± 2.59%), versus National variety, HAN (E_max = 43.80 ± 6.32%), MAN (E_max = 14.62 ± 1.69%), HFN (E_max = 64.87 ± 3.04%), and MFN (E_max = 31.01 ± 3.92%). Chromatographic fingerprints of HFV and HFN were found to have some similar signals that belong to monoterpenes, whereas for HAN and HAV similar signals were found belonging to fatty acids and triterpenoids. Methanolic extracts showed signals of (1) furfural, (2) furfural acetone (3) furfuraldehyde and (4) β-sitosterol compounds. Flavedo portion of C. sinensis possessed spasmolytic effect on rat ileum strips and antibacterial activity against Salmonella strains. This species is source for obtaining bioactive compounds with therapeutic potential in the treatment of infectious diarrhea.