Mediator lipidomics by liquid chromatography-tandem mass spectrometry

Yoshihiro Kita; Suzumi M Tokuoka; Takao Shimizu

doi:10.1016/j.bbalip.2017.03.008

Mediator lipidomics by liquid chromatography-tandem mass spectrometry

Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Aug;1862(8):777-781. doi: 10.1016/j.bbalip.2017.03.008. Epub 2017 Mar 27.

Authors

Yoshihiro Kita¹, Suzumi M Tokuoka², Takao Shimizu³

Affiliations

¹ Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Life Sciences Core Facility, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: kita@m.u-tokyo.ac.jp.
² Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
³ Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Lipid Signaling, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.

PMID: 28347870
DOI: 10.1016/j.bbalip.2017.03.008

Abstract

Comprehensive quantitative analysis of lipid mediators using liquid chromatography-tandem mass spectrometry is an effective strategy in the elucidation of disease mechanisms; but technically, it has been and is still a great challenge to achieve reliable datasets that cover variety of lipid metabolites contained at trace levels in complex biological matrices. In this opinion article, we introduce our experiences in developing lipid mediator profiling systems, and deliver some comments on limitations of current methodology.

Keywords: Cross-platform; Eicosanoids; Mass spectrometer; Quantification.

Publication types

Review

MeSH terms

Animals
Chromatography, Liquid / methods
Humans
Lipid Metabolism / physiology*
Lipids / chemistry*
Mass Spectrometry / methods
Metabolomics / methods
Tandem Mass Spectrometry / methods

Substances

Lipids