Mechanistic details of methanol oxidation catalyzed by the periplasmically-located pyrroloquinoline quinone-dependent methanol dehydrogenase of methylotrophs can be elucidated using site-directed mutants. Here, we present an in situ colony assay of methanol dehydrogenase, which allows robotic screening of large populations of intact small colonies, and regrowth of colonies for subsequent analysis.
Keywords: Dye-linked assay; Methanol dehydrogenase; Methylotrophs; PQQ; Pyrroloquinoline quinone; Robotic screening.
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