Microsequential injection lab-on-valve system for the spectrophotometric bi-parametric determination of iron and copper in natural waters

Alba González; Raquel B R Mesquita; Jessica Avivar; Tânia Moniz; Maria Rangel; Víctor Cerdà; António O S S Rangel

doi:10.1016/j.talanta.2017.02.055

Microsequential injection lab-on-valve system for the spectrophotometric bi-parametric determination of iron and copper in natural waters

Talanta. 2017 May 15:167:703-708. doi: 10.1016/j.talanta.2017.02.055. Epub 2017 Mar 1.

Authors

Alba González¹, Raquel B R Mesquita², Jessica Avivar³, Tânia Moniz⁴, Maria Rangel⁵, Víctor Cerdà⁶, António O S S Rangel⁷

Affiliations

¹ Group of Analytical Chemistry, Automation and Environment, Department of Chemistry, University of the Balearic Islands, E-07122 Palma de Mallorca, Spain.
² Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, R. Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal. Electronic address: rmesquita@porto.ucp.pt.
³ Sciware Systems, S.L. Spin-off of the University of the Balearic Islands, E-07193 Bunyola, Spain.
⁴ REQUIMTE-UCIBIO, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, 4169-007 Porto, Portugal.
⁵ REQUIMTE-UCIBIO, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal.
⁶ Group of Analytical Chemistry, Automation and Environment, Department of Chemistry, University of the Balearic Islands, E-07122 Palma de Mallorca, Spain; Sciware Systems, S.L. Spin-off of the University of the Balearic Islands, E-07193 Bunyola, Spain.
⁷ Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, R. Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.

PMID: 28340782
DOI: 10.1016/j.talanta.2017.02.055

Abstract

The determination of iron and copper exploiting a microsequential injection lab-on-valve system with online spectrophotometric detection is described. A new, environmental friendly 3-hydroxy-4-pyridinone chelator, functionalized with a polyethylene glycol chain (MRB12) to improve water solubility, was used for iron determination. For copper determination, 1-(2-pyridylazo)-2-naphthol (PAN) was used. Different parameters affecting the formation of the complexes were studied, namely sample volume, reagent concentration, and buffer composition and concentration. The optimized conditions, 150µL of sample volume and 250mgL^-1 of MRB12 for iron determination, and 200µL of sample and 120mgL^-1 of PAN for copper determination, enabled an LOD of 15 and 18µgL^-1 for iron and copper, respectively. The robustness of the developed procedure was assessed by the calculation of the relative standard deviation (RSD), 5% for iron determination and 2% for copper determination. The accuracy of the method was assessed comparing the results with two certified samples (RD<7.5%) and calculating recovery percentages with five river water samples (average<107%).

Keywords: 1-(2-pyridylazo)-2-naphthol; Bi-parametric determination; Iron and copper; PEGylated 3-hydroxy-4-pyridinone; Water analysis.

MeSH terms

Copper / analysis*
Flow Injection Analysis / instrumentation*
Flow Injection Analysis / methods
Fresh Water / analysis*
Iron / analysis*
Naphthols / chemistry
Polyethylene Glycols / chemistry
Pyridones / chemistry
Rivers / chemistry*
Spectrophotometry / methods*

Substances

Naphthols
Pyridones
1-(2-pyridylazo)-2-naphthol
3-hydroxy-4-pyridone
Polyethylene Glycols
Copper
Iron