Are cells from a snowman realistic? Cryopreserved tissues as a source for single-cell RNA-sequencing experiments

Felipe A Vieira Braga; Sarah A Teichmann; Michael J T Stubbington

doi:10.1186/s13059-017-1192-4

Are cells from a snowman realistic? Cryopreserved tissues as a source for single-cell RNA-sequencing experiments

Genome Biol. 2017 Mar 24;18(1):54. doi: 10.1186/s13059-017-1192-4.

Authors

Felipe A Vieira Braga¹, Sarah A Teichmann^{2

3

4}, Michael J T Stubbington⁵

Affiliations

¹ Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. fvb@sanger.ac.uk.
² Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.
³ European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SD, UK.
⁴ Cavendish Laboratory, Cambridge University, JJ Thomson Avenue, Cambridge, CB3 0HE, UK.
⁵ Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. ms31@sanger.ac.uk.

Abstract

A recently published study in Genome Biology shows that cells isolated from cryopreserved tissues are a reliable source of genetic material for single-cell RNA-sequencing experiments.Please see related Method article: http://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1171-9.

Publication types

Review
Comment

MeSH terms

Animals
Cryopreservation*
Humans
RNA / genetics
Sequence Analysis, RNA*
Single-Cell Analysis / methods*

Substances

RNA