Detection of Polish clinical Aspergillus fumigatus isolates resistant to triazoles

Urszula Nawrot; Ewelina Kurzyk; Maiken Cavling Arendrup; Martyna Mroczynska; Katarzyna Wlodarczyk; Beata Sulik-Tyszka; Marta Wróblewska; Marek Ussowicz; Przemyslaw Zdziarski; Kinga Niewinska; Anna Brillowska-Dabrowska

doi:10.1093/mmy/myx012

Detection of Polish clinical Aspergillus fumigatus isolates resistant to triazoles

Med Mycol. 2018 Jan 1;56(1):121-124. doi: 10.1093/mmy/myx012.

Affiliations

¹ Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 213, 50-566 Wroclaw, Poland.
² Department of Molecular Biotechnology and Microbiology, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland.
³ Unit of Mycology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, Denmark.
⁴ Department of Clinical Microbiology, Rigshospitalet, Copenhagen University, Copenhagen, Denmark.
⁵ Department of Microbiology, Central Clinical Hospital in Warsaw, Medical University of Warsaw, Banacha 1A, 02-097 Warsaw, Poland.
⁶ Department of Dental Microbiology, Medical University of Warsaw, Banacha 1A, 02-097 Warsaw, Poland.
⁷ Department of Pediatric Bone Marrow Transplantation, Oncology and Hematology, Wroclaw Medical University Borowska 213, 50-556 Wroclaw, Poland.
⁸ Department of Immunology and Infectious Diseases, Institute of Immunology and Experimental Therapy, Weigla 12, 53-114 Wroclaw, Poland.
⁹ Department of Anesthesiology and Intensive Therapy, Paediatric Intensive Care Unit Wroclaw Medical University, Chalubinskiego 1A, 50-368 Wroclaw, Poland.

PMID: 28340159
DOI: 10.1093/mmy/myx012

Abstract

We studied the presence of triazole resistance of 121 Aspergillus fumigatus clinical isolates collected in two Polish cities, Warsaw and Wrocław, to determine if resistance is emerging in our country. We identified five itraconazole resistant isolates (4.13%) carrying the TR34/L98H alteration in Cyp51A gene, four of which were cross-resistant to posaconazole and one to voriconazole. One isolate was intermediate susceptible to itraconazole and harbored no Cyp51A alterations. The study confirms the presence of azole resistant A. fumigatus strains in Poland at a level that is comparative to other European countries.

Keywords: Aspergillus fumigatus; TR34/L98H; azole resistance; environmental genotype; mutations in Cyp51A.

© The Author 2017. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

MeSH terms

Antifungal Agents / pharmacology*
Aspergillosis / microbiology*
Aspergillus fumigatus / drug effects*
Aspergillus fumigatus / isolation & purification*
Cities
Cytochrome P-450 Enzyme System / genetics
Drug Resistance, Fungal*
Fungal Proteins / genetics
Genotype
Humans
Mutation, Missense
Poland
Triazoles / pharmacology*

Substances

Antifungal Agents
Fungal Proteins
Triazoles
Cytochrome P-450 Enzyme System
cytochrome P-450 CYP51A, Aspergillus