Draft genome sequence of Thermoanaerobacterium sp. strain PSU-2 isolated from thermophilic hydrogen producing reactor

Sompong O-Thong; Peerawat Khongkliang; Chonticha Mamimin; Apinya Singkhala; Poonsuk Prasertsan; Nils-Kåre Birkeland

doi:10.1016/j.gdata.2017.02.012

Draft genome sequence of Thermoanaerobacterium sp. strain PSU-2 isolated from thermophilic hydrogen producing reactor

Genom Data. 2017 Feb 28:12:49-51. doi: 10.1016/j.gdata.2017.02.012. eCollection 2017 Jun.

Authors

Sompong O-Thong¹, Peerawat Khongkliang², Chonticha Mamimin², Apinya Singkhala², Poonsuk Prasertsan³, Nils-Kåre Birkeland⁴

Affiliations

¹ Research Center in Energy and Environment, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand; Biotechnology Program, Department of Biology, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand.
² Biotechnology Program, Department of Biology, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand.
³ Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112, Thailand.
⁴ Department of Biology and Centre for Geobiology, University of Bergen, P.O. Box 7800, N-5020 Bergen, Norway.

Abstract

Thermoanaerobacterium sp. strain PSU-2 was isolated from thermophilic hydrogen producing reactor and subjected to draft genome sequencing on 454 pyrosequencing and annotated on RAST. The draft genome sequence of strain PSU-2 contains 2,552,497 bases with an estimated G + C content of 35.2%, 2555 CDS, 8 rRNAs and 57 tRNAs. The strain had a number of genes responsible for carbohydrates metabolic, amino acids and derivatives, and protein metabolism of 17.7%, 14.39% and 9.81%, respectively. Strain PSU-2 also had gene responsible for hydrogen biosynthesis as well as the genes related to Ni-Fe hydrogenase. Comparative genomic analysis indicates strain PSU-2 shares about 94% genome sequence similarity with Thermoanaerobacterium xylanolyticum LX-11. The nucleotide sequence of this draft genome was deposited into DDBJ/ENA/GenBank under the accession MSQD00000000.

Keywords: Hydrogen producing bacteria; Thermoanaerobacterium sp.; Thermophile; Whole genome sequencing.