DNA nanostructures constructed with multi-stranded motifs

Donglei Yang; Zhenyu Tan; Yongli Mi; Bryan Wei

doi:10.1093/nar/gkx121

DNA nanostructures constructed with multi-stranded motifs

Nucleic Acids Res. 2017 Apr 7;45(6):3606-3611. doi: 10.1093/nar/gkx121.

Authors

Donglei Yang¹, Zhenyu Tan², Yongli Mi^{1

3}, Bryan Wei²

Affiliations

¹ School of Chemical Science and Engineering, Tongji University, Shanghai 200092, China.
² School of Life Sciences, Tsinghua University-Peking University Center for Life Sciences, Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China.
³ Department of Chemical and Biomolecular Engineering, the Hong Kong University of Science and Technology, Kowloon, Hong Kong.

Abstract

Earlier studies in DNA self-assembly have foretold the feasibility of building addressable nanostructures with multi-stranded motifs, which is fully validated in this study. In realizing this feasibility in DNA nanotechnology, a diversified set of motifs of modified domain lengths is extended from a classic type. The length of sticky ends can be adjusted to form different dihedral angles between the matching motifs, which corresponds to different connecting patterns. Moreover, the length of rigidity core can also be tuned to result in different dihedral angles between the component helices of a certain motif therefore different numbers of component helices. The extended set of motifs is used for self-assembly of complex one dimensional, two dimensional and three dimensional structures.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry*
Models, Molecular
Nanostructures / chemistry*
Nanostructures / ultrastructure
Nanotechnology
Nucleic Acid Conformation

Substances

DNA