Effect of monomer blend and chlorhexidine-adding on physical, mechanical and biological properties of experimental infiltrants

Luciana Tiemi Inagaki; Roberta Caroline Bruschi Alonso; Giovana Albamonte Spagnolo Araújo; Eduardo José Carvalho de Souza-Junior; Paula Cristina Anibal; José Francisco Höfling; Fernanda Miori Pascon; Regina Maria Puppin-Rontani

doi:10.1016/j.dental.2016.09.028

Effect of monomer blend and chlorhexidine-adding on physical, mechanical and biological properties of experimental infiltrants

Dent Mater. 2016 Dec;32(12):e307-e313. doi: 10.1016/j.dental.2016.09.028. Epub 2016 Oct 28.

Authors

Luciana Tiemi Inagaki¹, Roberta Caroline Bruschi Alonso², Giovana Albamonte Spagnolo Araújo³, Eduardo José Carvalho de Souza-Junior⁴, Paula Cristina Anibal⁵, José Francisco Höfling⁶, Fernanda Miori Pascon⁷, Regina Maria Puppin-Rontani⁸

Affiliations

¹ Piracicaba Dental School, Department of Pediatric Dentistry, P.O. BOX 52, Piracicaba 13414-903, SP, Brazil. Electronic address: luciana.inagaki@gmail.com.
² Anhanguera University of São Paulo, Biomaterials Department, Rua Maria Cândida, 1813, Bloco G - 6° andar, São Paulo 02071-013, SP, Brazil. Electronic address: robalonso@yahoo.com.
³ Piracicaba Dental School, Department of Restorative Dentistry, Dental Materials Division, P.O. BOX 52, Piracicaba 13414-903, SP, Brazil. Electronic address: giovanaaraujo@hotmail.com.
⁴ University Center of Várzea Grande, Prosthodontic and Dental Materials Department, Várzea Grande, Mato Grosso, Brazil Av Dom Orlando Chaves, 2655, Bairro Cristo Rei - Varzea Grande, 78118-900, MT, Brazil. Electronic address: edujcsj@gmail.com.
⁵ Piracicaba Dental School, Department of Oral Diagnostic, Laboratory of Microbiology and Immunology, P.O. BOX 52, Piracicaba 13414-903, SP, Brazil. Electronic address: anibal.paula_c@yahoo.com.br.
⁶ Piracicaba Dental School, Department of Oral Diagnostic, Laboratory of Microbiology and Immunology, P.O. BOX 52, Piracicaba 13414-903, SP, Brazil. Electronic address: hofling@fop.unicamp.br.
⁷ Piracicaba Dental School, Department of Pediatric Dentistry, P.O. BOX 52, Piracicaba 13414-903, SP, Brazil. Electronic address: fmpascon@fop.unicamp.br.
⁸ Piracicaba Dental School, Department of Pediatric Dentistry, P.O. BOX 52, Piracicaba 13414-903, SP, Brazil. Electronic address: rmpuppin@fop.unicamp.br.

PMID: 28327302
DOI: 10.1016/j.dental.2016.09.028

Abstract

Objectives: The aim of the study was to evaluate antimicrobial inhibition zone, degree of conversion (DC) and Knoop hardness (KH) of experimental infiltrants. Experimental low viscosity monomer blends were prepared and chlorhexidine diacetate salt (CHX) (0.1% or 0.2%) was added comprising the groups: G1) TEGDMA; G2) TEGDMA/0.1CHX; G3) TEGDMA/0.2CHX; G4) TEGDMA/UDMA; G5) TEGDMA/UDMA/0.1CHX; G6) TEGDMA/UDMA/0.2CHX; G7) TEGDMA/BISEMA; G8) TEGDMA/BISEMA/0.1CHX; G9) TEGDMA/BISEMA/0.2CHX. Icon^® was used as control group.

Methods: Specimens of resin blends were made (n=9) to accomplished DC and KH. Pour plate was accomplished to evaluate antimicrobial groups' activity against Streptococcus mutans (SM) and Lactobacillus acidophilus (LA). Data obtained were submitted to two-way ANOVA and Tukey tests for blends comparisons and Dunnett's test for comparisons between experimental infiltrants and Icon^® (p<0.05).

Results: In relation to antimicrobial effect, uncured blends showed higher antibacterial activity than cured ones for the most of blends. After polymerization, G5 showed the highest inhibition zone against SM and, G3 and G6 against LA. Concerning KH, TEGDMA/UDMA-based blends showed the highest values of KH number and it was influenced by monomeric base, regardless CHX concentration. DC was not affected by monomer blend composition, neither for CHX concentration. The antimicrobial activity was affected by monomeric base, CHX concentration and polymerization. Experimental infiltrants presented similar or higher performance than Icon^® for the properties evaluated.

Significance: White spot lesion infiltration with low viscosity monomer blends (infiltrants) is an alternative to stop initial caries lesions progression. The incorporation of an antimicrobial agent as chlorhexidine diacetate salt in infiltrants composition could enhance the performance of these materials.

Keywords: Antimicrobial activity; BISEMA; Chlorhexidine; Degree of conversion; Knoop hardness; TEGDMA; UDMA.

MeSH terms

Chlorhexidine*
Composite Resins*
Hardness
Materials Testing
Polymerization

Substances

Composite Resins
Chlorhexidine