Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP)

Feng Liu; Xuyang Feng; Wenbin Ma

doi:10.1007/978-1-4939-6892-3_19

Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP)

Methods Mol Biol. 2017:1587:205-214. doi: 10.1007/978-1-4939-6892-3_19.

Authors

Feng Liu¹, Xuyang Feng¹, Wenbin Ma²

Affiliations

¹ Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, 132 East Waihuan Rd., Higher Education Mega Center, Guangzhou, People's Republic of China.
² Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, 132 East Waihuan Rd., Higher Education Mega Center, Guangzhou, People's Republic of China. mawenbin@mail.sysu.edu.cn.

PMID: 28324511
DOI: 10.1007/978-1-4939-6892-3_19

Abstract

Telomere chromatin immunoprecipitation (ChIP) is an experimental method used to determine whether proteins are associated with telomere DNA inside the nuclei of cells and tissues. Telomere-associated proteins are first covalently crosslinked to telomere DNA, and then immunoprecipitated using an antibody specific for the protein of interest. This method has become one of the most indispensable tools for investigating the protein complexes that associate with telomeres.

Keywords: ChIP; Telomere chromatin immunoprecipitation; Telomere-associated proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatin / genetics*
Chromatin Immunoprecipitation / methods
DNA / genetics
DNA-Binding Proteins / genetics
Humans
Protein Binding / genetics
Telomere / genetics*

Substances

Chromatin
DNA-Binding Proteins
DNA