Telomere Length Analysis by Quantitative Fluorescent in Situ Hybridization (Q-FISH)

Isabelle Ourliac-Garnier; Arturo Londoño-Vallejo

doi:10.1007/978-1-4939-6892-3_3

Telomere Length Analysis by Quantitative Fluorescent in Situ Hybridization (Q-FISH)

Methods Mol Biol. 2017:1587:29-39. doi: 10.1007/978-1-4939-6892-3_3.

Authors

Isabelle Ourliac-Garnier^{1

2}, Arturo Londoño-Vallejo^{3

4}

Affiliations

¹ Telomeres & Cancer laboratory, CNRS-UMR3244, Institut Curie, 26 rue d'Ulm, 75248, Paris, France.
² UPMC Univ. Paris 06, F-75005, Paris, France.
³ Telomeres & Cancer laboratory, CNRS-UMR3244, Institut Curie, 26 rue d'Ulm, 75248, Paris, France. arturo.londono@curie.fr.
⁴ UPMC Univ. Paris 06, F-75005, Paris, France. arturo.londono@curie.fr.

PMID: 28324495
DOI: 10.1007/978-1-4939-6892-3_3

Abstract

Length is a functional parameter of telomeres, the nucleoprotein structures that protect chromosome ends. The availability of highly specific, high affinity probes for telomeric repeat sequences allowed the development of quantitative approaches aimed at measuring telomere length directly on chromosomes or in interphase nuclei. Here, we describe a general method for telomere quantitative FISH on metaphase chromosomes and discuss its most common applications in research.

Keywords: FISH; LNA; Length; PNA; Q-FISH; Telomere.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes / genetics
Humans
In Situ Hybridization, Fluorescence / methods
Interphase / genetics
Metaphase / genetics
Repetitive Sequences, Nucleic Acid / genetics
Telomere / genetics*