The development of techniques and methods for allergen purification is essential for diagnosis and the development of safe immunotherapeutic agents. The most common purification techniques include chromatographic methodologies. In this chapter, we review and describe the details of the methodologies of using ion-exchange, gel-filtration, and affinity chromatography to purify two well-known panallergens, profilin and parvalbumin.
Keywords: Allergen extraction; Allergen purification; Allergy; Chromatographic techniques; Chromatography; Parvalbumin; Profilin.