RNA interference knockdown of aminopeptidase N genes decrease the susceptibility of Chilo suppressalis larvae to Cry1Ab/Cry1Ac and Cry1Ca-expressing transgenic rice

Lin Qiu; Jinxing Fan; Boyao Zhang; Lang Liu; Xiaoping Wang; Chaoliang Lei; Yongjun Lin; Weihua Ma

doi:10.1016/j.jip.2017.03.001

RNA interference knockdown of aminopeptidase N genes decrease the susceptibility of Chilo suppressalis larvae to Cry1Ab/Cry1Ac and Cry1Ca-expressing transgenic rice

J Invertebr Pathol. 2017 May:145:9-12. doi: 10.1016/j.jip.2017.03.001. Epub 2017 Mar 6.

Authors

Lin Qiu¹, Jinxing Fan², Boyao Zhang², Lang Liu², Xiaoping Wang², Chaoliang Lei², Yongjun Lin³, Weihua Ma⁴

Affiliations

¹ National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Wuhan, China; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
² Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
³ National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Wuhan, China.
⁴ National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Wuhan, China; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: weihuama@mail.hzau.edu.cn.

PMID: 28274717
DOI: 10.1016/j.jip.2017.03.001

Abstract

Transgenic rice expressing Bacillus thuringiensis (Bt) Cry toxins are resistant to lepidopteran pests, such as Chilo suppressalis, a major insect pest of rice in Asia. Understanding how these toxins interact with their hosts is crucial to understanding their insecticidal action. In this study, knockdown of two aminopeptidase N genes (APN1 and APN2) by RNA interference resulted in decreased susceptibility of C. suppressalis larvae to the Bt rice varieties TT51 (Cry1Ab and Cry1Ac fusion genes) and T1C-19 (Cry1Ca), but not T2A-1 (Cry2Aa). This suggests that APN1 and APN2 are receptors for Cry1A and Cry1C toxins in C. suppressalis.

Keywords: Aminopeptidase N; Bacillus thuringiensis; Bt receptor; Chilo suppressalis; Cry toxin; RNA interference.

MeSH terms

Animals
Bacillus thuringiensis Toxins
Bacterial Proteins / genetics
Endotoxins / genetics
Gene Knockdown Techniques
Genes, Insect / genetics
Hemolysin Proteins / genetics
Larva
Lepidoptera / genetics*
Oryza / genetics
Oryza / microbiology*
Pest Control, Biological / methods*
Plants, Genetically Modified / genetics*

Substances

Bacillus thuringiensis Toxins
Bacterial Proteins
Endotoxins
Hemolysin Proteins
insecticidal crystal protein, Bacillus Thuringiensis