A TaqMan-based qRT-PCR assay for Senecavirus A detection in tissue samples of neonatal piglets

Alais Maria Dall Agnol; Rodrigo Alejandro Arellano Otonel; Raquel Arruda Leme; Amauri Alcindo Alfieri; Alice Fernandes Alfieri

doi:10.1016/j.mcp.2017.03.002

A TaqMan-based qRT-PCR assay for Senecavirus A detection in tissue samples of neonatal piglets

Mol Cell Probes. 2017 Jun:33:28-31. doi: 10.1016/j.mcp.2017.03.002. Epub 2017 Mar 4.

Authors

Alais Maria Dall Agnol¹, Rodrigo Alejandro Arellano Otonel², Raquel Arruda Leme¹, Amauri Alcindo Alfieri³, Alice Fernandes Alfieri¹

Affiliations

¹ Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil; Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil.
² Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil.
³ Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil; Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil. Electronic address: alfieri@uel.br.

PMID: 28267624
DOI: 10.1016/j.mcp.2017.03.002

Abstract

This study describes a sensitive (1.3 × 10¹ genomic copies/μL) and specific TaqMan-based qRT-PCR assay able to detect and quantify SVA RNA in porcine biological samples. The technique represents an efficient tool for the virus diagnosis and assessment of SVA load in tissues of infected animals and for epidemiological studies.

Keywords: Picornavirus infection; Quantitative PCR; Real-time RT-PCR; Seneca valley virus; Swine; Vesicular disease.

MeSH terms

Animals
Animals, Newborn / genetics
Animals, Newborn / virology
Picornaviridae / genetics*
Picornaviridae / isolation & purification*
Picornaviridae / pathogenicity
Picornaviridae Infections / diagnosis*
Picornaviridae Infections / genetics*
Picornaviridae Infections / veterinary
Picornaviridae Infections / virology
Swine / genetics
Swine / virology

Supplementary concepts

Senecavirus A