Graphene oxide-enhanced cytoskeleton imaging and mitosis tracking

Qian-Ru Li; Jin-Biao Jiao; Li-Li Li; Xiao-Peng He; Yi Zang; Tony D James; Guo-Rong Chen; Lin Guo; Jia Li

doi:10.1039/c7cc01019b

Graphene oxide-enhanced cytoskeleton imaging and mitosis tracking

Chem Commun (Camb). 2017 Mar 16;53(23):3373-3376. doi: 10.1039/c7cc01019b.

Authors

Qian-Ru Li¹, Jin-Biao Jiao², Li-Li Li³, Xiao-Peng He², Yi Zang³, Tony D James⁴, Guo-Rong Chen², Lin Guo¹, Jia Li³

Affiliations

¹ State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, P. R. China. guol@whu.edu.cn.
² Key Laboratory for Advanced Materials & Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China. xphe@ecust.edu.cn.
³ National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 189 Guo Shoujing Rd., Shanghai 201203, P. R. China. yzang@simm.ac.cn jli@simm.ac.cn.
⁴ Department of Chemistry, University of Bath, Bath, BA2 7AY, UK.

PMID: 28265597
DOI: 10.1039/c7cc01019b

Abstract

Here we show that graphene oxide greatly enhances the imaging ability of a peptide probe that selectively targets microtubules of the cytoskeleton, thus enabling the dynamic tracking of mitosis in live cells.

MeSH terms

Amino Acid Sequence
Cytoskeleton / metabolism*
Fluorescence
Fluorescent Dyes / chemistry
Fluorescent Dyes / metabolism*
Fluorescent Dyes / toxicity
Graphite / chemistry
Graphite / metabolism*
Graphite / toxicity
HeLa Cells
Humans
Limit of Detection
Microscopy, Confocal
Microtubules / chemistry
Microtubules / metabolism
Mitosis*
Molecular Imaging
Peptides / chemistry
Peptides / metabolism*
Peptides / toxicity
Rhodamines / chemistry
Rhodamines / metabolism
Rhodamines / toxicity

Substances

5-carboxytetramethylrhodamine
Fluorescent Dyes
Peptides
Rhodamines
Graphite