Abstract
Leukemia cells from a patient with chronic lymphocytic leukemia (CLL) were found to bind sheep RBC (SRBC) through their monoclonal surface IgM. A lymphoblastoid cell line was obtained by immortalization of leukemic cells with Epstein-Barr virus (EBV). Cultured leukemic cells were found to have a supernumerary chromosome 12, an abnormality typical of CLL of the B cell type. To our knowledge, this is the first EBV-immortalized cell line from B-CLL cells of known SRBC specificity and the third reported CLL cell line carrying trisomy of chromosome 12.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology*
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Antibodies, Neoplasm / immunology*
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Antigens, Surface / immunology
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B-Lymphocytes / immunology*
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B-Lymphocytes / ultrastructure
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Cell Line
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Cell Transformation, Viral
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Chromosomes, Human, Pair 12*
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Erythrocytes / immunology
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Female
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Herpesvirus 4, Human
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Humans
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Immunoglobulin M / immunology*
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Leukemia, Lymphoid / genetics
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Leukemia, Lymphoid / immunology
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Leukemia, Lymphoid / pathology*
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Middle Aged
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Rosette Formation
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Sheep / immunology
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Trisomy
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Tumor Cells, Cultured / immunology*
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Tumor Cells, Cultured / ultrastructure
Substances
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Antibodies, Monoclonal
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Antibodies, Neoplasm
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Antigens, Surface
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Immunoglobulin M