Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone

Mehdi Kazemzadeh-Narbat; Jeroen Rouwkema; Nasim Annabi; Hao Cheng; Masoumeh Ghaderi; Byung-Hyun Cha; Mansi Aparnathi; Akbar Khalilpour; Batzaya Byambaa; Esmaiel Jabbari; Ali Tamayol; Ali Khademhosseini

doi:10.1002/adhm.201601122

Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone

Adv Healthc Mater. 2017 May;6(10). doi: 10.1002/adhm.201601122. Epub 2017 Feb 27.

Authors

Mehdi Kazemzadeh-Narbat^{1

2}, Jeroen Rouwkema^{1

2

3}, Nasim Annabi^{1

2

4

5}, Hao Cheng^{1

2}, Masoumeh Ghaderi^{1

2}, Byung-Hyun Cha^{1

2}, Mansi Aparnathi^{1

2}, Akbar Khalilpour^{1

2}, Batzaya Byambaa^{1

2}, Esmaiel Jabbari⁶, Ali Tamayol^{1

2

4}, Ali Khademhosseini^{1

2

4

7

8}

Affiliations

¹ Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.
² Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
³ Department of Biomechanical Engineering, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, 7522NB, Enschede, The Netherlands.
⁴ Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
⁵ Department of Chemical Engineering, Northeastern University, Boston, MA, 02115-5000, USA.
⁶ Biomimetic Materials and Tissue Engineering Laboratories, Department of Chemical Engineering, University of South Carolina, Columbia, SC, 29208, USA.
⁷ Department of Physics, King Abdulaziz University, Jeddah, 21569, Saudi Arabia.
⁸ Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Seoul, 05029, Republic of Korea.

PMID: 28240417
DOI: 10.1002/adhm.201601122

Abstract

Engineering bone tissue requires the generation of a highly organized vasculature. Cellular behavior is affected by the respective niche. Directing cellular behavior and differentiation for creating mineralized regions surrounded by vasculature can be achieved by controlling the pattern of osteogenic and angiogenic niches. This manuscript reports on engineering vascularized bone tissues by incorporating osteogenic and angiogenic cell-laden niches in a photocrosslinkable hydrogel construct. Two-step photolithography process is used to control the stiffness of the hydrogel and distribution of cells in the patterned hydrogel. In addittion, osteoinductive nanoparticles are utilized to induce osteogenesis. The size of microfabricated constructs has a pronounced effect on cellular organization and function. It is shown that the simultaneous presence of both osteogenic and angiogenic niches in one construct results in formation of mineralized regions surrounded by organized vasculature. In addition, the presence of angiogenic niche improves bone formation. This approach can be used for engineered constructs that can be used for treatment of bone defects.

Keywords: bone tissue engineering; hydrogels; micropatterning; vascularization.

MeSH terms

Animals
Bone Regeneration
Humans
Hydrogels / chemistry*
Nanoparticles / chemistry
Osteogenesis / physiology
Tissue Engineering / methods

Substances

Hydrogels