Visualization of Mesenchymal Stromal Cells in 2Dand 3D-Cultures by Scanning Electron Microscopy with Lanthanide Contrasting

I A Novikov; I V Vakhrushev; E N Antonov; K N Yarygin; A M Subbot

doi:10.1007/s10517-017-3659-4

Visualization of Mesenchymal Stromal Cells in 2Dand 3D-Cultures by Scanning Electron Microscopy with Lanthanide Contrasting

Bull Exp Biol Med. 2017 Feb;162(4):558-562. doi: 10.1007/s10517-017-3659-4. Epub 2017 Feb 27.

Authors

I A Novikov¹, I V Vakhrushev², E N Antonov³, K N Yarygin², A M Subbot⁴

Affiliations

¹ Institute of Eye Diseases, Moscow, Russia.
² V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
³ Institute on Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia.
⁴ Institute of Eye Diseases, Moscow, Russia. kletkagb@gmail.com.

PMID: 28239796
DOI: 10.1007/s10517-017-3659-4

Abstract

Mesenchymal stromal cells from deciduous teeth in 2D- and 3D-cultures on culture plastic, silicate glass, porous polystyrene, and experimental polylactoglycolide matrices were visualized by scanning electron microscopy with lanthanide contrasting. Supravital staining of cell cultures with a lanthanide-based dye (neodymium chloride) preserved normal cell morphology and allowed assessment of the matrix properties of the carriers. The developed approach can be used for the development of biomaterials for tissue engineering.

Keywords: lanthanide; mesenchymal stromal cells; scanning electron microscopy; tissue engineering.

MeSH terms

Biocompatible Materials / chemistry
Biocompatible Materials / pharmacology
Cell Culture Techniques
Cell Differentiation / drug effects
Cell Proliferation / drug effects
Contrast Media / chemistry*
Dental Pulp / cytology
Dental Pulp / drug effects
Glass / chemistry
Humans
Mesenchymal Stem Cells / drug effects
Mesenchymal Stem Cells / ultrastructure*
Microscopy, Electron, Scanning / methods*
Neodymium / chemistry*
Osteogenesis
Plastics / chemistry
Plastics / pharmacology
Polyesters / chemistry
Polyesters / pharmacology
Polystyrenes / chemistry
Polystyrenes / pharmacology
Primary Cell Culture
Staining and Labeling / methods
Tissue Engineering
Tissue Scaffolds / chemistry*
Tooth, Deciduous / cytology
Tooth, Deciduous / drug effects

Substances

Biocompatible Materials
Contrast Media
Plastics
Polyesters
Polystyrenes
neodymium chloride
Neodymium
poly(lactide)