Optical control of cell signaling by single-chain photoswitchable kinases

Science. 2017 Feb 24;355(6327):836-842. doi: 10.1126/science.aah3605.

Abstract

Protein kinases transduce signals to regulate a wide array of cellular functions in eukaryotes. A generalizable method for optical control of kinases would enable fine spatiotemporal interrogation or manipulation of these various functions. We report the design and application of single-chain cofactor-free kinases with photoswitchable activity. We engineered a dimeric protein, pdDronpa, that dissociates in cyan light and reassociates in violet light. Attaching two pdDronpa domains at rationally selected locations in the kinase domain, we created the photoswitchable kinases psRaf1, psMEK1, psMEK2, and psCDK5. Using these photoswitchable kinases, we established an all-optical cell-based assay for screening inhibitors, uncovered a direct and rapid inhibitory feedback loop from ERK to MEK1, and mediated developmental changes and synaptic vesicle transport in vivo using light.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans
  • Cell Line, Tumor
  • Drug Evaluation, Preclinical
  • Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors*
  • Extracellular Signal-Regulated MAP Kinases / chemistry
  • Extracellular Signal-Regulated MAP Kinases / genetics
  • Extracellular Signal-Regulated MAP Kinases / radiation effects*
  • Humans
  • Light
  • MAP Kinase Kinase 1 / antagonists & inhibitors*
  • MAP Kinase Kinase 1 / chemistry
  • MAP Kinase Kinase 1 / genetics
  • MAP Kinase Kinase 1 / radiation effects*
  • Mutation
  • Protein Domains
  • Protein Engineering
  • Protein Kinase Inhibitors / chemistry
  • Protein Kinase Inhibitors / pharmacology*
  • Protein Multimerization
  • Signal Transduction

Substances

  • Protein Kinase Inhibitors
  • Extracellular Signal-Regulated MAP Kinases
  • MAP Kinase Kinase 1