Isoquinoline alkaloids from Coptis japonica stimulate the myoblast differentiation via p38 MAP-kinase and Akt signaling pathway

Heyjin Lee; Le Thi Tuong; Ji Hye Jeong; Sang-Jin Lee; Gyu-Un Bae; Jae-Ha Ryu

doi:10.1016/j.bmcl.2017.02.003

Isoquinoline alkaloids from Coptis japonica stimulate the myoblast differentiation via p38 MAP-kinase and Akt signaling pathway

Bioorg Med Chem Lett. 2017 Mar 15;27(6):1401-1404. doi: 10.1016/j.bmcl.2017.02.003. Epub 2017 Feb 4.

Authors

Heyjin Lee¹, Le Thi Tuong¹, Ji Hye Jeong¹, Sang-Jin Lee¹, Gyu-Un Bae¹, Jae-Ha Ryu²

Affiliations

¹ Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 100 Chungparo 47-Gil, Yongsan-Gu, Seoul 04310, Republic of Korea.
² Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 100 Chungparo 47-Gil, Yongsan-Gu, Seoul 04310, Republic of Korea. Electronic address: ryuha@sookmyung.ac.kr.

PMID: 28228365
DOI: 10.1016/j.bmcl.2017.02.003

Abstract

To overcome the muscle atrophy, such as cachexia and sarcopenia, we tried to find myogenic agents from medicinal plants. From myogenic extract of Coptis japonica, we purified six isoquinoline alkaloids and evaluated their effects on transactivation of myoD and MHC expression in C2C12 cells during differentiation process. Among obtained compounds, magnoflorine most efficiently enhanced the myoblast differentiation by activating the p38 MAP kinase and Akt pathway, and also increased the number of multinucleated and cylinder-shaped myotubes. These results propose that magnoflorine from Coptis japonica might be a promising lead compound for the development of anti-muscle atrophy drug.

Keywords: Akt; Coptis japonica; Isoquinoline alkaloid; MyoD; Myoblast differentiation; p38 MAPK.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkaloids / pharmacology*
Animals
Cell Differentiation / drug effects*
Cell Line
Coptis / chemistry*
Mice
Myoblasts / cytology
Myoblasts / drug effects*

Substances

Alkaloids