Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

Hamid Soleimaninejad; Moore Z Chen; Xiaoding Lou; Trevor A Smith; Yuning Hong

doi:10.1039/c6cc09916e

Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

Chem Commun (Camb). 2017 Mar 2;53(19):2874-2877. doi: 10.1039/c6cc09916e.

Authors

Hamid Soleimaninejad¹, Moore Z Chen¹, Xiaoding Lou², Trevor A Smith¹, Yuning Hong³

Affiliations

¹ School of Chemistry, The University of Melbourne, Parkville VIC 3010, Australia. trevoras@unimelb.edu.au.
² Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, P. R. China.
³ School of Chemistry, The University of Melbourne, Parkville VIC 3010, Australia. trevoras@unimelb.edu.au and Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia. Y.Hong@latrobe.edu.au.

PMID: 28220157
DOI: 10.1039/c6cc09916e

Abstract

We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells. An amine-reactive aggregation-induced emission fluorogen is used to label proteins in the cytoplasm and the change in the protein mobility as well as local viscosity can be monitored by using fluorescence anisotropy imaging and fluorescence lifetime imaging, respectively.

MeSH terms

Amines / chemical synthesis
Amines / chemistry*
Animals
Cell Line
Fluorescence Polarization*
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Macromolecular Substances / chemistry
Mice
Molecular Structure

Substances

Amines
Fluorescent Dyes
Macromolecular Substances