Genome-wide identification of GLABRA3 downstream genes for anthocyanin biosynthesis and trichome formation in Arabidopsis

Biochem Biophys Res Commun. 2017 Apr 1;485(2):360-365. doi: 10.1016/j.bbrc.2017.02.074. Epub 2017 Feb 17.

Abstract

GLABRA3 (GL3), a bHLH transcription factor, has previously proved to be involved in anthocyanin biosynthesis and trichome formation in Arabidopsis, however, its downstream targeted genes are still largely unknown. Here, we found that GL3 was widely present in Arabidopsis vegetative and reproductive organs. New downstream targeted genes of GL3 for anthocyanin biosynthesis and trichome formation were identified in young shoots and expanding true leaves by RNA sequencing. GL3-mediated gene expression was tissue specific in the two biological processes. This study provides new clues to further understand the GL3-mediated regulatory network of anthocyanin biosynthesis and trichome formation in Arabidopsis.

Keywords: Anthocyanin biosynthesis; Arabidopsis; GL3; Trichome formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthocyanins / biosynthesis*
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Basic Helix-Loop-Helix Transcription Factors / genetics*
  • Basic Helix-Loop-Helix Transcription Factors / metabolism
  • Gene Expression Profiling / methods
  • Gene Expression Regulation, Plant*
  • Gene Regulatory Networks / genetics
  • Genome, Plant / genetics
  • Mutation
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Shoots / genetics
  • Plant Shoots / metabolism
  • Plants, Genetically Modified
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, RNA / methods
  • Signal Transduction / genetics
  • Trichomes / genetics*
  • Trichomes / metabolism

Substances

  • Anthocyanins
  • Arabidopsis Proteins
  • Basic Helix-Loop-Helix Transcription Factors
  • GL3 protein, Arabidopsis