Receptor-based screening assays for the detection of antibiotics residues - A review

Saeed Ahmed; Jianan Ning; Guyue Cheng; Ijaz Ahmad; Jun Li; Liu Mingyue; Wei Qu; Mujahid Iqbal; M A B Shabbir; Zonghui Yuan

doi:10.1016/j.talanta.2017.01.057

Receptor-based screening assays for the detection of antibiotics residues - A review

Talanta. 2017 May 1:166:176-186. doi: 10.1016/j.talanta.2017.01.057. Epub 2017 Jan 21.

Authors

Saeed Ahmed¹, Jianan Ning¹, Guyue Cheng², Ijaz Ahmad³, Jun Li¹, Liu Mingyue¹, Wei Qu², Mujahid Iqbal¹, M A B Shabbir¹, Zonghui Yuan⁴

Affiliations

¹ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China.
² MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan 430070, China.
³ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China; The University of Agriculture Peshawar, Pakistan.
⁴ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China; MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: yuan5802@mail.hzau.edu.cn.

PMID: 28213220
DOI: 10.1016/j.talanta.2017.01.057

Abstract

Consumer and regulatory agencies have a high concern to antibiotic residues in food producing animals, so appropriate screening assays of fast, sensitive, low cost, and easy sample preparation for the identification of these residues are essential for the food-safety insurance. Great efforts in the development of a high-throughput antibiotic screening assay have been made in recent years. Concerning the screening of antibiotic residue, this review elaborate an overview on the availability, advancement and applicability of antibiotic receptor based screening assays for the safety assessment of antibiotics usage (i.e. radio receptor assay, enzyme labeling assays, colloidal gold receptor assay, enzyme colorimetry assay and biosensor assay). This manuscript also tries to shed a light on the selection, preparation and future perspective of receptor protein for antibiotic residue detection. These assays have been introduced for the screening of numerous food samples. Receptor based screening technology for antibiotic detection has high accuracy. It has been concluded that at the same time, it can detect a class of drugs for certain receptor, and realize the multi-residue detection. These assays offer fast, easy and precise detection of antibiotics.

Keywords: Antibiotics; Labeling technique; Receptor; Residue; Screening assays.

Publication types

Review

MeSH terms

Animals
Anti-Bacterial Agents / analysis*
Anti-Bacterial Agents / metabolism
Chemistry Techniques, Analytical / methods*
Drug Residues / analysis*
Drug Residues / metabolism
Humans
Receptors, Cell Surface / metabolism*

Substances

Anti-Bacterial Agents
Receptors, Cell Surface