ABC-Transporter Mediated Sterol Export from Cells Using Radiolabeled Sterols

Alryel Yang; Ingrid C Gelissen

doi:10.1007/978-1-4939-6875-6_20

ABC-Transporter Mediated Sterol Export from Cells Using Radiolabeled Sterols

Methods Mol Biol. 2017:1583:275-285. doi: 10.1007/978-1-4939-6875-6_20.

Authors

Alryel Yang¹, Ingrid C Gelissen²

Affiliations

¹ Faculty of Pharmacy, The University of Sydney, Pharmacy Bank Building A15, Camperdown, Sydney, NSW, 2006, Australia.
² Faculty of Pharmacy, The University of Sydney, Pharmacy Bank Building A15, Camperdown, Sydney, NSW, 2006, Australia. Ingrid.gelissen@sydney.edu.au.

PMID: 28205181
DOI: 10.1007/978-1-4939-6875-6_20

Abstract

Cholesterol export from cells to extracellular acceptors represents the first step of the reverse cholesterol transport process and is an essential part of the multifaceted pathway for cells to control their cholesterol levels. Malfunction of this pathway leads to cholesterol accumulation in cells such as macrophages, which can form the basis of conditions like atherosclerosis. A number of ATP-binding cassette (ABC) transporters, namely ABCA1, ABCA7, ABCG1, and ABCG4, play an essential role in this process. In this chapter, we describe methods utilizing radiolabeled sterols for measuring ABC-transporter mediated sterol export, utilizing endogenously expressed transporters as well as overexpression systems.

Keywords: ABC-transporter; ABCA1; ABCA7; ABCG1; ABCG4; Cholesterol export; Reverse cholesterol transport.

MeSH terms

ATP-Binding Cassette Transporters / genetics
ATP-Binding Cassette Transporters / metabolism*
Animals
Atherosclerosis / genetics
Atherosclerosis / metabolism
Biological Transport, Active / physiology
CHO Cells
Cholesterol / genetics
Cholesterol / metabolism*
Cricetulus
Humans
Isotope Labeling / methods*
THP-1 Cells

Substances

ATP-Binding Cassette Transporters
Cholesterol