Objective: To investigate the effect of atopy on the expression of glucocorticoid receptors in children with bronchiolitis.
Methods: ELISA was used to measure the changes in the serum levels of glucocorticoid receptor α (GRα) and glucocorticoid receptor β (GRβ) in the bronchiolitis group (77 children, including 34 children with atopy) and pneumonia group (68 children). Thirty-eight children who were prepared to undergo surgeries for non-infectious diseases and had no atopy or family history of allergic diseases were enrolled as the control group.
Results: The bronchiolitis group and the pneumonia group had significant increases in the serum levels of GRα and GRβ compared with the control group (P<0.01), and the bronchiolitis group had significant increases in these levels compared with the pneumonia group (P<0.01). Compared with the control group and the pneumonia group, the bronchiolitis group had a significant increase in the GRα/GRβ ratio (P<0.01). Compared with the control group, the children with or without atopy in the bronchiolitis group had significant increases in the serum levels of GRα and GRβ (P<0.01). The non-atopic children in the bronchiolitis group had a significant increase in the serum level of GRβ compared with the atopic children (P<0.01). The atopic children in the bronchiolitis group had a significant increase in the GRα/GRβ ratio compared with the control group and non-atopic children in the bronchiolitis group (P<0.01).
Conclusions: Children with bronchiolitis have increased serum levels of GRα and GRβ. The children with atopy have an increased GRα/GRβ ratio, suggesting that the atopic children with bronchiolitis are highly sensitive to glucocorticoids.
目的: 探讨特应性体质（简称特应质）对毛细支气管炎（简称毛支）患儿糖皮质激素受体（GR）表达的影响。
方法: 采用ELISA法测定和比较毛支组（77例，包括34例特应质患儿）和肺炎组（68例）患儿血清中GRα、GRβ水平的变化。38例同期住院的非感染性小儿外科术前患儿，且无特应性及过敏性疾病家族史的患儿作为对照组。
结果: 与对照组比较，毛支组和肺炎组中血清GRα、GRβ水平均明显升高（P < 0.01）；毛支组血清GRα、GRβ水平高于肺炎组（P < 0.01）。毛支组GRα/GRβ比值较对照组和肺炎组明显升高（P < 0.01）。毛支组特应质和非特应质患儿血清GRα、GRβ水平与对照组比较均明显升高（P < 0.01）；毛支组非特应质患儿GRβ水平明显高于特应质患儿（P < 0.01）；毛支组特应质患儿GRα/GRβ比值较对照组和毛支组非特应质患儿明显升高（P < 0.01）。
结论: 毛支患儿血清GRα、GRβ表达水平增强；特应质毛支患儿表现为GRα/GRβ比值增加，提示特应质毛支患儿对糖皮质激素有较高的敏感性。