Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs

Bernadeth F Ticar; Zuliyati Rohmah; Solange I Mussatto; Jae-Min Lim; Seongha Park; Byeong-Dae Choi

doi:10.1016/j.carbpol.2016.12.065

Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs

Carbohydr Polym. 2017 Apr 1:161:16-20. doi: 10.1016/j.carbpol.2016.12.065. Epub 2016 Dec 29.

Authors

Bernadeth F Ticar¹, Zuliyati Rohmah², Solange I Mussatto³, Jae-Min Lim⁴, Seongha Park⁴, Byeong-Dae Choi⁵

Affiliations

¹ Department of Seafood and Aquaculture Science, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-gil, Tongyeong, Gyeongnam, 650-160, South Korea; College of Arts and Sciences, Iloilo Science and Technology University, Burgos St., La Paz, Iloilo City, 5000, Philippines. Electronic address: berna2423@gmail.com.
² Department of Seafood and Aquaculture Science, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-gil, Tongyeong, Gyeongnam, 650-160, South Korea; Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
³ Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800, Kongens Lyngby, Denmark.
⁴ Department of Chemistry, Changwon National University, Changwon, 53210, South Korea.
⁵ Department of Seafood and Aquaculture Science, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-gil, Tongyeong, Gyeongnam, 650-160, South Korea.

PMID: 28189224
DOI: 10.1016/j.carbpol.2016.12.065

Abstract

Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The electrospray ionization tandem mass spectrometry (ESI-MS/MS) confirmed the presence of uronic acids on F3, which could be a ^0,2A₂ fragment plus loss of methyl group which is very common among non-methylated, sulfated disaccharides.

Keywords: Hyaluronic acid; Hyaluronidase inhibitory effect; L. tessellatus eggs.

MeSH terms

Animals
Enzyme Activation / drug effects
Glycosaminoglycans / isolation & purification
Glycosaminoglycans / pharmacology*
Hyaluronoglucosaminidase / antagonists & inhibitors*
Ovum / chemistry*
Perciformes*
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry

Substances

Glycosaminoglycans
Hyaluronoglucosaminidase