Multiclonal Expansion of Klebsiella pneumoniae Isolates Producing NDM-1 in Rio de Janeiro, Brazil

Antimicrob Agents Chemother. 2017 Mar 24;61(4):e01048-16. doi: 10.1128/AAC.01048-16. Print 2017 Apr.

Abstract

We characterized NDM-1-producing Klebsiella isolates from Rio de Janeiro, Brazil. PCR was applied for resistance and virulence determinants. The genetic context of blaNDM was determined by S1 nuclease pulsed-field gel electrophoresis (PFGE) and hybridization. Genotyping was performed by PFGE and multilocus sequence typing (MLST). Most isolates carried multiple resistance genes and remained susceptible to amikacin, fosfomycin-trometamol, polymyxin B, and tigecycline. The spread of NDM-1-producing Klebsiella pneumoniae was not associated with clonal expansion and appears to be associated with Tn3000.

Keywords: Klebsiella pneumoniae; MLST; NDM carbapenemase; New Delhi metallo-β-lactamase; molecular typing.

MeSH terms

  • Amikacin / pharmacology
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Typing Techniques
  • Brazil / epidemiology
  • Clone Cells
  • DNA Transposable Elements*
  • Drug Resistance, Multiple, Bacterial / genetics*
  • Electrophoresis, Gel, Pulsed-Field
  • Fosfomycin / pharmacology
  • Gene Expression
  • Genotype
  • Humans
  • Klebsiella Infections / epidemiology
  • Klebsiella Infections / microbiology
  • Klebsiella Infections / transmission
  • Klebsiella pneumoniae / classification
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Minocycline / analogs & derivatives
  • Minocycline / pharmacology
  • Multilocus Sequence Typing
  • Phylogeny
  • Plasmids / chemistry
  • Plasmids / metabolism
  • Polymyxin B / pharmacology
  • Tigecycline
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • DNA Transposable Elements
  • Fosfomycin
  • Tigecycline
  • Amikacin
  • beta-Lactamases
  • beta-lactamase NDM-1
  • Minocycline
  • Polymyxin B