Abstract
We characterized NDM-1-producing Klebsiella isolates from Rio de Janeiro, Brazil. PCR was applied for resistance and virulence determinants. The genetic context of blaNDM was determined by S1 nuclease pulsed-field gel electrophoresis (PFGE) and hybridization. Genotyping was performed by PFGE and multilocus sequence typing (MLST). Most isolates carried multiple resistance genes and remained susceptible to amikacin, fosfomycin-trometamol, polymyxin B, and tigecycline. The spread of NDM-1-producing Klebsiella pneumoniae was not associated with clonal expansion and appears to be associated with Tn3000.
Keywords:
Klebsiella pneumoniae; MLST; NDM carbapenemase; New Delhi metallo-β-lactamase; molecular typing.
Copyright © 2017 American Society for Microbiology.
MeSH terms
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Amikacin / pharmacology
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Anti-Bacterial Agents / pharmacology*
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Bacterial Typing Techniques
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Brazil / epidemiology
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Clone Cells
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DNA Transposable Elements*
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Drug Resistance, Multiple, Bacterial / genetics*
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Electrophoresis, Gel, Pulsed-Field
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Fosfomycin / pharmacology
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Gene Expression
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Genotype
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Humans
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Klebsiella Infections / epidemiology
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Klebsiella Infections / microbiology
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Klebsiella Infections / transmission
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Klebsiella pneumoniae / classification
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Klebsiella pneumoniae / drug effects
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Klebsiella pneumoniae / genetics*
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Klebsiella pneumoniae / isolation & purification
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Minocycline / analogs & derivatives
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Minocycline / pharmacology
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Multilocus Sequence Typing
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Phylogeny
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Plasmids / chemistry
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Plasmids / metabolism
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Polymyxin B / pharmacology
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Tigecycline
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
Substances
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Anti-Bacterial Agents
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DNA Transposable Elements
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Fosfomycin
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Tigecycline
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Amikacin
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beta-Lactamases
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beta-lactamase NDM-1
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Minocycline
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Polymyxin B