The miR-599 promotes non-small cell lung cancer cell invasion via SATB2

Biochem Biophys Res Commun. 2017 Mar 25;485(1):35-40. doi: 10.1016/j.bbrc.2017.02.005. Epub 2017 Feb 4.

Abstract

MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. Here, we identified that miR-599 is up-regulated in non-small cell lung cancer (NSCLC) patients. It promoted NSCLC cell proliferation by negatively regulating SATB2. In NSCLC cell lines, CCK-8 proliferation assay indicated that the cell proliferation is promoted by miR-599 mimics. Transwell assay showed that miR-599 mimics promoted the invasion and migration of NSCLC cells. Luciferase assays confirmed that miR-599 directly binds to the 3'untranslated region of SATB2, and western blotting showed that miR-599 suppresses the expression of SATB2 at the protein level. This study indicates that miR-599 promotes proliferation and invasion of NSCLC cell lines via SATB2. The miR-599 may represent a potential therapeutic target for NSCLC treatment.

Keywords: NSCLC; SATB2; miR-599.

Publication types

  • Research Support, Non-U.S. Gov't
  • Retracted Publication

MeSH terms

  • 3' Untranslated Regions
  • Aged
  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Carcinoma, Non-Small-Cell Lung / pathology
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Lung / metabolism
  • Lung / pathology*
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology
  • Male
  • Matrix Attachment Region Binding Proteins / genetics*
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness / genetics
  • Neoplasm Invasiveness / pathology
  • Transcription Factors / genetics*
  • Up-Regulation

Substances

  • 3' Untranslated Regions
  • MIRN599 microRNA, human
  • Matrix Attachment Region Binding Proteins
  • MicroRNAs
  • SATB2 protein, human
  • Transcription Factors