Determination of histamine in fish by Surface Enhanced Raman Spectroscopy using silver colloid SERS substrates

Tibor Janči; Davor Valinger; Jasenka Gajdoš Kljusurić; Lara Mikac; Sanja Vidaček; Mile Ivanda

doi:10.1016/j.foodchem.2016.12.032

Determination of histamine in fish by Surface Enhanced Raman Spectroscopy using silver colloid SERS substrates

Food Chem. 2017 Jun 1:224:48-54. doi: 10.1016/j.foodchem.2016.12.032. Epub 2016 Dec 16.

Authors

Tibor Janči¹, Davor Valinger², Jasenka Gajdoš Kljusurić³, Lara Mikac⁴, Sanja Vidaček⁵, Mile Ivanda⁶

Affiliations

¹ Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. Electronic address: tjanci@pbf.hr.
² Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. Electronic address: dvalinger@pbf.hr.
³ Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. Electronic address: jgajdos@pbf.hr.
⁴ Center of Excellence for Advanced Materials and Sensing Devices, Ruđer Bošković Institute, Bijenička c. 54, 10000 Zagreb, Croatia. Electronic address: lmikac@irb.hr.
⁵ Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. Electronic address: svidacek@pbf.hr.
⁶ Center of Excellence for Advanced Materials and Sensing Devices, Ruđer Bošković Institute, Bijenička c. 54, 10000 Zagreb, Croatia. Electronic address: ivanda@irb.hr.

PMID: 28159292
DOI: 10.1016/j.foodchem.2016.12.032

Abstract

This study was focused on development of a rapid and sensitive method for histamine determination in fish based on Surface Enhanced Raman Spectroscopy (SERS) using simple and widely available silver colloid SERS substrate. Extraction of histamine with 0.4M perchloric acid and purification with 1-butanol significantly shortened sample preparation (30min) and provided clear SERS spectra with characteristic Raman bands of histamine. Principal component analysis effectively distinguished SERS spectra of fish samples with different histamine content. Partial least square (PLS) regression models confirmed reliability of detection and spectral analysis of histamine with SERS. In histamine concentration range 0-200mgkg^-1, significant in legislative and fish quality control aspects, PLS regression model based on spectral range 1139.9-1643.7cm^-1 showed linear trend with R²_pred=0.962, RPD=7.250. Presented protocol for histamine extraction and purification followed by SERS analysis coupled with chemometric approach, enabled development of rapid and inexpensive method for histamine determination in fish.

Keywords: Fish; Histamine; PCA; PLS; Raman spectroscopy; SERS; Silver colloid.

MeSH terms

Animals
Food Analysis / methods*
Histamine / analysis*
Perciformes*
Reproducibility of Results
Seafood / analysis*
Silver
Spectrum Analysis, Raman / methods*

Substances

colloidal silver
Silver
Histamine