Regenerated surface-enhanced Raman scattering (SERS) substrates allow users the ability to not only reuse sensing surfaces, but also tailor them to the sensing application needs (wavelength of the available laser, plasmon band matching). In this review, we discuss the development of SERS substrates for response to emerging threats and some of our collaborative efforts to improve on the use of commercially available substrate surfaces. Thus, we are able to extend the use of these substrates to broader Army needs (like emerging threat response).